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2:18
YouTube
Awal Sutamihardja
Thick Blood Smear for Giemsa Malaria Microscopy, Part 1 of 3
Thick Blood Smear for Giemsa Malaria Microscopy: This is a video presentation on how to make a Thick Blood Smear (TBS) for Giemsa malaria microscopy. TBS made from 10ul whole blood in EDTA tube, size 1x2 cm rectangle. Dry the TBS on slide warmer 37'C for 15 minutes. Fresh Giemsa solution 4%, 45minutes staining. Buffer PBS 1x, pH 7.2. Rinse the ...
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Perfect Blood Smear & Staining Guide Approximately 10 µl of whole blood, after the blood smear is prepared, place it in a well-ventilated area to dry for later use. Pay attention to the following points when staining: 1. The blood smear must be completely dry before starting the staining process. 2. The slide should be placed horizontally, evenly sprinkle 0.5 ml of Solution A and 1 ml of Solution B over the entire slide, use a rubber bulb to ensure thorough mixing and stain for 4 minutes (6–7 mi
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