Plasmids comprising transgene insertions in four lines of transgenic mice have been retrieved by plasmid rescue into a set of Escherichia coli strains with mutations in different members of the ...

Researchers have developed PlasmidMaker, a versatile automated platform for plasmid design & construction. These circular DNA molecules are used by scientists to introduce new genes into a target ...

Genetic engineering of mammalian cells with multiple transgenes is an essential need in contemporary cell biology. Examples include the reprogramming of somatic cells into stem cells by coexpression ...

Electrophoretic analysis of EcoRI and HindIII restriction fragments of 2-μ m supercoiled DNA of Saccharomyces cerevisiae indicated that this class of DNA is heterogeneous and probably consists of two ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...

The use of restriction enzymes to characterize DNA has been popular since the 1970s. Today, this technique remains one of the easiest and fastest ways to evaluate DNA sequences. As with most ...