The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

GeneFisher—fishing for genes, perhaps? Fishing genes out of genomes? Just fishing? The subtitle, Interactive PCR Primer Design, “hooked me” (get it?) but it shouldn’t have in retrospect. After all, ...

Polymerase chain reaction (PCR) and rapid (antigen) tests for COVID-19 both involve taking a swab. Results for a PCR test take longer, as it needs to go to a laboratory. It is more costly but tends to ...

Co-Diagnostics (CODX) has performed an in silico analysis of the primers sets used in its Co-Primers based PCR tests for the detection of ...

Abundance, richness, and diversity of fungi in Urtica dioica roots for the three primer pairs tested fITS7/ITS4, gITS7/ITS4, and 5.8S-Fun/ITS4-fun (Ta = 57 °C), the addition of PNA clamps (+PNA) and ...

Seroepidemiological studies have shown that approximately 60% of adults in developed countries and up to 100% of adults in developing countries are positive for antibodies against HCMV. 5 BMT ...

COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes–an ...