mito-QC illuminates mitophagy and mitochondrial architecture …
WEBAug 1, 2016 · Using confocal microscopy, we demonstrate that mito-QC is compatible with classical and contemporary techniques in histochemistry and allows unambiguous in vivo detection of mitophagy and mitochondrial morphology at single-cell resolution within multiple organ systems.
mito-QC illuminates mitophagy and mitochondrial architecture in …
WEBAug 1, 2016 · Although the mt-Keima mouse is undoubtedly a powerful model, mito-QC overcomes these limitations and represents an unrivalled and experimentally advantageous tool that can unequivocally resolve multiple aspects of mitochondrial cell biology in vivo at an unprecedented cellular resolution.
The mito-QC Reporter for Quantitative Mitophagy Assessment in …
WEBMar 10, 2020 · We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito -QC mice to quantify mitophagy activation in vitro and ex vivo.
Seeing is believing: a novel tool for quantitating mitophagy
WEBJun 19, 2020 · Mito-SRAI is a tandem construct containing a MMTS and two fluorescent protein tags, YPet and TOLLES. When mitochondria are healthy, mito-SRAI emits both yellow and cyan fluorescence in ...
Investigating Mitophagy and Mitochondrial Morphology In Vivo
WEBWe recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts.
Semi-automated quantitation of mitophagy in cells and tissues
WEBThe mito-QC Counter is a new semi-automated tool to quantify mitophagy. •. Mitophagy is induced in ARPE19 cells and varies in distinct skeletal muscle regions. Abbreviations: DFP, deferiprone; ROI, region of interest; stdDev, standard deviation; TEM, …
Mitochondrial fission and mitophagy are independent …
WEBMay 12, 2021 · Therefore, we developed a model to classify distinct mitochondrial morphologies in our MitoQC mice (Fig. 3A, Supplementary Material, and Supplementary Fig. 1).
The mito -QC Reporter for Quantitative Mitophagy Assessment in …
WEBMar 10, 2020 · We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito -QC mice to quantify mitophagy activation in vitro and ex vivo.
Mito-QC illuminates mitophagy and mitochondrial architecture …
WEBJul 25, 2016 · Strikingly, our model uncovers highly enriched and differential zones of mitophagy in the developing heart and within specific cells of the adult kidney. mito -QC is an experimentally...
Mitophagy curtails cytosolic mtDNA-dependent activation of
WEBJan 27, 2024 · In the present study, we assessed mitophagy using the fixable mito-QC reporter C57BL/6J mouse 14, which ubiquitously expresses a chimeric protein that consists of pH-insensitive mCherry,...